Perineuronal nets in neurodegeneration

At the end of the 19 th century, Camillo Golgi discovered that neurons were enwrapped by a net, but it was only recently that neuroscientists started to intensely investigate the role of this mysterious reticular structure. Today, perineuronal nets (PNNs) are known to be lattice-like extracellular matrix aggregates surrounding synapses at the soma, proximal axons and dendrites of several types of neurons, including fast-spiking GABAergic interneurons, pyramidal neurons, cerebellar Purkinje cells and spinal cord motoneurons. PNNs are composed of chondroitin sulfate proteoglycans (CSGPs, including aggrecan, neurocan and brevican), hyaluronan, tenascins and link proteins. They first appear at the end of the critical period of neurodevelopment when they are thought to contribute to the constitution and stabilization of synapses around mature postsynaptic neurons. In adults, PNNs are stable and restrict neuroplasticity, but have an ability to undergo dynamic changes in response to learning and memory, stress, activation of the immune system, or CNS injuries and diseases. This remodeling is achieved by CSPG-degrading proteases, such as matrix metalloproteinases and ADAMTS proteoglycanases (a disintegrin and metalloproteinase with thrombospondin motifs) [1]. Alterations in the PNN structure have been observed in several CNS diseases, including psychiatric disorders, Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS). Recent studies have shown that a loss of PNNs is associated with neurodegeneration and progression of ALS-related symptoms in rodents over-expressing the human superoxide dismutase SOD1 G93A mutation. Forostyak and colleagues reported that the PNNs surrounding cervical and lumbar spinal cord motoneurons of SOD1 G93A rats are considerably degraded by the end-stage of the disease when compared to wild-type rats. Interestingly, they demonstrated that transplantation of mesenchymal stromal cells from the bone marrow partially prevents ALS-related degradation of PNNs in SOD1 G93A rats, thereby increasing motoneuron survival and lifespan [2]. Similarly, we recently showed a decrease in PNNs around lumbar spinal cord motoneurons of SOD1 G93A mice at the symptomatic stage. The expression and proteolytic activity of ADAMTS-4, the highest expressed ADAMTS proteoglycanase in the CNS, were progressively decreased in the spinal cord during advancement of ALS pathology. Also, when active recombinant human ADAMTS-4 was intracerebroventricularly injected into SOD1 G93A mice before the disease onset, the ALS-induced degradation of PNNs was associated with enhanced neurodegeneration and neuromuscular dysfunctions. The study indicated that decreased endogenous activity of ADAMTS-4 may be an adaptive attempt to preserve integrity of PNNs in ALS [3]. Altogether, these studies suggest that the disorganization/ degradation of PNNs …